Research and Development (Subsurface Microbiology Research Group)
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Northern Advancement Center for Science & Technology
H-RISE
5-3 Sakaemachi, Horonobe-cho, Teshio-gun, Hokkaido, 098-3221, Japan.
TEL+81-1632-9-4112
FAX +81-1632-9-4113
H-RISE
5-3 Sakaemachi, Horonobe-cho, Teshio-gun, Hokkaido, 098-3221, Japan.
TEL+81-1632-9-4112
FAX +81-1632-9-4113
Research Contents
1. Researches on field science for better understanding of subsurface environment for diatomaceous formation and coal beds.
Estimation and assessment of subsurface microbial ecosystem by developing a phylogenetic database for microorganisms living in diatomaceous formation and coal beds.

Research Topics & Publications
- Ueno A, Shimizu S, Tamamura S, Naganuma T, Ohmi Y, Kaneko K. (2014) Structural alteration of humic acids by Pseudomonas spp. from deep terrestrial subsurface diatomite formations in northernmost Japan. Geomicrobiology Journal 31:654-663
- Shimizu S, Ueno A, Tamamura S, Naganuma T, Kaneko K (2013) Methanoculleus horonobensis sp. nov., a methanogenic archaeon isolated from a deep diatomaceous shale formation. International Journal of Systematic and Evolutionary Microbiology 63: 4320–4323 (adopted in cover photo).
- Shimizu S, Upadhye R, Ishijima Y, Naganuma T (2011) Methanosarcina horonobensis sp. nov., a methanogenic archaeon isolated from a deep subsurface Miocene formation. International Journal of Systematic and Evolutionary Microbiology 61:2503–2507.
- Shimizu S, Ueno A, Ishijima Y (2011) Microbial Communities Associated with Acetate-Rich Gas-Petroleum Reservoir Surface Facilities. Bioscience, Biotechnology, and Biochemistry 75:1835-1837.

Research Activities & Column
- 2019/09/04JHSS35 is held in Wakkanai, Hokkaido, Japan.
- 2018/06/11【Poster presentation】Our recent study has been reported in Pyro2018, Kyoto, Japan.
- 2018/05/23[Poster presentation] Presentation in Pyro2018
- 2016/08/10【Oral presentation】Oral presentation in IHSS18.
- 2016/04/01【Poster presentation】Our recent study has been reported in JSBBA2016, Sapporo, Japan.

Major Equipments
- 1. Anaerobic Chambers (Hirasawa Co., Ltd)
- 2. Deoxygenized Gas Pressure & Replace Injector:GR-8 (SANSHIN INDUSTRIAL Co., Ltd / GR-8)
- 3. Gas Chromatograph-Mass Spectrometry (JEOL Co. Ltd / JMS-Q1050GC)
- 4. Liquid Chromatography (SHIMADZU Co. Ltd / LC20A)
- 5. Gas Chromatography (SHIMADZU Co. Ltd / GC-8A, GC-14B, GC-2010 Plus)
