Subsurface Microbiology Research Group | H-RISE Northern Advancement Center for Science & Technology Horonobe Research Institute for the Subsurface Environment

Research and Development (Subsurface Microbiology Research Group)

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Northern Advancement Center for Science & Technology
H-RISE
5-3 Sakaemachi, Horonobe-cho, Teshio-gun, Hokkaido, 098-3221, Japan.
TEL+81-1632-9-4112
FAX +81-1632-9-4113

Research Contents

1. Research on element technology for development of methane gas deposit and CO2 storage using the subsurface environment.

Exploration for methane-producing microbial communities in a subsurface environment in diatomaceous formation and coal beds, and identifying their functions.

Identification of the methanogenesis process in subsurface environment and evaluation of the application method.

Exploration for microorganisms capable of producing organic matter from CO2 stored in a subsurface environment, identification of their functions, and evaluation of the utilization method.

Research Topics & Publications

Ueno A, Shimizu S, Hashimoto M, Adachi T, Matsushita T, Okuyama H, Yoshida K (2017) Effects of aerobic growth on the fatty acid and hydrocarbon compositions of Geobacter bemidjiensis Bem^T. Journal of Oleo Science 66:93-101.
Ueno A, Shimizu S, Tamamura S, Okuyama H, Naganuma T, Kaneko K. (2016) Anaerobic decomposition of humic substances by Clostridium from the deep subsurface. Scientific Reports 6:18990
Shimizu S, Ueno A, Naganuma T, Kaneko, K (2015) Methanosarcina subterranea sp. nov., a methanogenic archaeon isolated from a deep subsurface diatomaceous shale formation. International Journal of Systematic and Evolutionary Microbiology 65, 1167-1171
Ueno A, Shimizu S, Tamamura S, Naganuma T, Ohmi Y, Kaneko K. (2014) Structural alteration of humic acids by Pseudomonas spp. from deep terrestrial subsurface diatomite formations in northernmost Japan. Geomicrobiology Journal 31:654-663
Shimizu S, Ueno A, Tamamura S, Naganuma T, Kaneko K (2013) Methanoculleus horonobensis sp. nov., a methanogenic archaeon isolated from a deep diatomaceous shale formation. International Journal of Systematic and Evolutionary Microbiology 63: 4320–4323 (adopted in cover photo).
Shimizu S, Ueno A, Ishijima Y (2011) Microbial Communities Associated with Acetate-Rich Gas-Petroleum Reservoir Surface Facilities. Bioscience, Biotechnology, and Biochemistry 75:1835-1837.
Shimizu S, Akiyama M, Naganuma T, Fujioka M, Nako M, Ishijima Y (2007) Molecular characterization of microbial communities in deep coal seam groundwater of northern Japan. Geobiology 5:423-433.

Research Activities & Column

Major Equipments

1. Anaerobic Chambers (Hirasawa Co., Ltd)
2. Deoxygenized Gas Pressure & Replace Injector:GR-8 (SANSHIN INDUSTRIAL Co., Ltd / GR-8)
3. Gas Chromatograph-Mass Spectrometry (JEOL Co. Ltd / JMS-Q1050GC)
4. Liquid Chromatography (SHIMADZU Co. Ltd / LC20A)
5. Gas Chromatography (SHIMADZU Co. Ltd / GC-8A, GC-14B, GC-2010 Plus)